Thanks Abhishek for the suggestion.....
let me tell you below points..
- there is no issue with TRs.. they all moves properly and objects are all in active state and process chains are also loading data.. only the problem is that we have to monitor the chain other wise it is not triggering informatica workflow.
- I have tried with multiple credential all the cases issue is same.
- here we have two connections one is SAP with informatica sand box. other one with the live system.
here I dont have problem with sandbox but only in live system we have issue. and as per my DBA team RFC connection is fine.